来自假单胞菌属的原儿茶酸3,4-双加氧酶（PCO）在与对羟基苯甲酸羟化酶偶联后可用于酶法测碱酯酶。在单分子实验中可改善有机荧光团稳定性 ，也可用于研究根瘤菌中原儿茶酸的代谢。

Specifications

Source：Recombinant from Bacteria

EC：1.13.11.3

Product Form：Solution

Specific Activity：> 3 Units / mg

Reaction Equation

3,4-Dihydroxybenzoate + O2 3-Carboxy-cis,cis-muconate

Contaminants

NADPH oxidase < 0.01 % 

Alkaline phosphatase < 0.002 %

Other Features

Gene: Protocatechuate 3,4-dioxygenase gene cloned from Acinetobacter baumannii

Production: Purified from the extract of transformed E.coli by conventional chromatographies

Purity: Single major band on SDS-PAGE (low contaminants from host E.coli)

Application: Useful for removal of protocatechuate derived from choline esterase determination

Stability: Wide range of pH stability from 5 to 10

Storage Condition：1 - 10°C (do not freeze)